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dna ligation kit ver 2 1  (TaKaRa)


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    Structured Review

    TaKaRa dna ligation kit ver 2 1
    Dna Ligation Kit Ver 2 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 587 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dna+ligation+kit/DNA+Ligation+Kit%2C+Version+2%2E1/pmc12805085-49-1-5
    Average 96 stars, based on 587 article reviews
    dna ligation kit ver 2 1 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: A single-nucleotide enhancer mutation overrides chromosomal sex to drive XX male development.
    Article Snippet: Enh13 was amplified along with the addition of restriction enzymes BglII and HindIII sites using 2X Phusion (NEB, M0531L, Primers used in Table S1). .. DNA inserts were digested using BglII and HindIII enzymes (Thermo, K1991) and ligated into the pGL4.26 plasmid (Promega) between the BglII and HindIII sites in the multiple cloning site using a DNA ligation kit (Takara, 6022). ..

    Article Title: A reabsorption mechanism in Drosophila renal tubules maintains amino acid homeostasis
    Article Snippet: .. Complementary oligonucleotides with overhangs were annealed and cloned into the BbsI-digested U6b vector using a DNA ligation kit (Takara Bio, 6023). ..

    Article Title: Identification of oxyresveratrol, a food-as-medicine substrate, as a novel EPI that targets the RND efflux pumps of Serratia surfactantfaciens sp. nov. YD25 T .
    Article Snippet: .. The knockout plasmid pKNOCK-Gm was also double digested and purified, and then connected with each insert and transformed using the DNA Ligation Kit (TaKaRa, China). ..

    Article Title: A single-nucleotide enhancer mutation overrides chromosomal sex to drive XX male development
    Article Snippet: Enh13 was amplified along with the addition of restriction enzymes BglII and HindIII sites using 2X Phusion (NEB, M0531L, Primers used in Table ). .. DNA inserts were digested using BglII and HindIII enzymes (Thermo, K1991) and ligated into the pGL4.26 plasmid (Promega) between the BglII and HindIII sites in the multiple cloning site using a DNA ligation kit (Takara, 6022). ..

    Article Title: BaEV-pseudotyped lentiviral vectors enable stable CAR expression and cytotoxic function in NK cells
    Article Snippet: All constructs were synthesized by Lugen Sci Co., Ltd. (Bucheon-si, Gyeonggi-do, Republic of Korea). .. Constructs were cloned into the XbaI and SalI restriction sites of the pCDH-MSCV vector (System Biosciences, Palo Alto, CA, USA) using a DNA Ligation Kit (#6023, Takara Bio, Shiga, Japan). ..

    Cloning:

    Article Title: A single-nucleotide enhancer mutation overrides chromosomal sex to drive XX male development.
    Article Snippet: Enh13 was amplified along with the addition of restriction enzymes BglII and HindIII sites using 2X Phusion (NEB, M0531L, Primers used in Table S1). .. DNA inserts were digested using BglII and HindIII enzymes (Thermo, K1991) and ligated into the pGL4.26 plasmid (Promega) between the BglII and HindIII sites in the multiple cloning site using a DNA ligation kit (Takara, 6022). ..

    Article Title: A single-nucleotide enhancer mutation overrides chromosomal sex to drive XX male development
    Article Snippet: Enh13 was amplified along with the addition of restriction enzymes BglII and HindIII sites using 2X Phusion (NEB, M0531L, Primers used in Table ). .. DNA inserts were digested using BglII and HindIII enzymes (Thermo, K1991) and ligated into the pGL4.26 plasmid (Promega) between the BglII and HindIII sites in the multiple cloning site using a DNA ligation kit (Takara, 6022). ..

    DNA Ligation:

    Article Title: A single-nucleotide enhancer mutation overrides chromosomal sex to drive XX male development.
    Article Snippet: Enh13 was amplified along with the addition of restriction enzymes BglII and HindIII sites using 2X Phusion (NEB, M0531L, Primers used in Table S1). .. DNA inserts were digested using BglII and HindIII enzymes (Thermo, K1991) and ligated into the pGL4.26 plasmid (Promega) between the BglII and HindIII sites in the multiple cloning site using a DNA ligation kit (Takara, 6022). ..

    Article Title: Optimized expression and antimicrobial activity of rainbow trout defensin 3 in Komagataella phaffii.
    Article Snippet: Restriction enzymes EcoRI, NotI, and SacI-HF were purchased from New England Biolabs (NEB, USA). .. The DL2000 DNA marker and DNA ligation kit were purchased from TaKaRa Biotechnology Co., Ltd. (Dalian, China). .. Low molecular weight pre-stained protein marker was obtained from Thermo Fisher Scientific (USA).

    Article Title: Computationally guided discovery of Ly6e/LY6E-dependent AAV capsid variants
    Article Snippet: PrimeSTAR® Max DNA Polymerase , TaKaRa Bio Inc , Cat# R045A. .. DNA Ligation Kit , TaKaRa Bio Inc , Cat# 6023. .. T4 Polynucleotide Kinase , TaKaRa Bio Inc , Cat# 2021S.

    Article Title: A reabsorption mechanism in Drosophila renal tubules maintains amino acid homeostasis
    Article Snippet: .. Complementary oligonucleotides with overhangs were annealed and cloned into the BbsI-digested U6b vector using a DNA ligation kit (Takara Bio, 6023). ..

    Article Title: Identification of oxyresveratrol, a food-as-medicine substrate, as a novel EPI that targets the RND efflux pumps of Serratia surfactantfaciens sp. nov. YD25 T .
    Article Snippet: .. The knockout plasmid pKNOCK-Gm was also double digested and purified, and then connected with each insert and transformed using the DNA Ligation Kit (TaKaRa, China). ..

    Article Title: A single-nucleotide enhancer mutation overrides chromosomal sex to drive XX male development
    Article Snippet: Enh13 was amplified along with the addition of restriction enzymes BglII and HindIII sites using 2X Phusion (NEB, M0531L, Primers used in Table ). .. DNA inserts were digested using BglII and HindIII enzymes (Thermo, K1991) and ligated into the pGL4.26 plasmid (Promega) between the BglII and HindIII sites in the multiple cloning site using a DNA ligation kit (Takara, 6022). ..

    Article Title: BaEV-pseudotyped lentiviral vectors enable stable CAR expression and cytotoxic function in NK cells
    Article Snippet: All constructs were synthesized by Lugen Sci Co., Ltd. (Bucheon-si, Gyeonggi-do, Republic of Korea). .. Constructs were cloned into the XbaI and SalI restriction sites of the pCDH-MSCV vector (System Biosciences, Palo Alto, CA, USA) using a DNA Ligation Kit (#6023, Takara Bio, Shiga, Japan). ..

    Article Title: Tracing the emergence of the novel fluoroquinolone resistance gene qrtA in enterococci through environmental reservoirs and pELF-type linear plasmids
    Article Snippet: .. The DNA fragments to be inserted were constructed by PCR using the respective primers and inserted into the pCJK47 vector using the restriction enzyme Eco RI (Roche) and a DNA ligation kit (Takara Bio, Shiga, Japan) as previously described (Table S8) . ..

    Marker:

    Article Title: Optimized expression and antimicrobial activity of rainbow trout defensin 3 in Komagataella phaffii.
    Article Snippet: Restriction enzymes EcoRI, NotI, and SacI-HF were purchased from New England Biolabs (NEB, USA). .. The DL2000 DNA marker and DNA ligation kit were purchased from TaKaRa Biotechnology Co., Ltd. (Dalian, China). .. Low molecular weight pre-stained protein marker was obtained from Thermo Fisher Scientific (USA).

    Clone Assay:

    Article Title: A reabsorption mechanism in Drosophila renal tubules maintains amino acid homeostasis
    Article Snippet: .. Complementary oligonucleotides with overhangs were annealed and cloned into the BbsI-digested U6b vector using a DNA ligation kit (Takara Bio, 6023). ..

    Article Title: BaEV-pseudotyped lentiviral vectors enable stable CAR expression and cytotoxic function in NK cells
    Article Snippet: All constructs were synthesized by Lugen Sci Co., Ltd. (Bucheon-si, Gyeonggi-do, Republic of Korea). .. Constructs were cloned into the XbaI and SalI restriction sites of the pCDH-MSCV vector (System Biosciences, Palo Alto, CA, USA) using a DNA Ligation Kit (#6023, Takara Bio, Shiga, Japan). ..

    Knock-Out:

    Article Title: Identification of oxyresveratrol, a food-as-medicine substrate, as a novel EPI that targets the RND efflux pumps of Serratia surfactantfaciens sp. nov. YD25 T .
    Article Snippet: .. The knockout plasmid pKNOCK-Gm was also double digested and purified, and then connected with each insert and transformed using the DNA Ligation Kit (TaKaRa, China). ..

    Purification:

    Article Title: Identification of oxyresveratrol, a food-as-medicine substrate, as a novel EPI that targets the RND efflux pumps of Serratia surfactantfaciens sp. nov. YD25 T .
    Article Snippet: .. The knockout plasmid pKNOCK-Gm was also double digested and purified, and then connected with each insert and transformed using the DNA Ligation Kit (TaKaRa, China). ..

    Transformation Assay:

    Article Title: Identification of oxyresveratrol, a food-as-medicine substrate, as a novel EPI that targets the RND efflux pumps of Serratia surfactantfaciens sp. nov. YD25 T .
    Article Snippet: .. The knockout plasmid pKNOCK-Gm was also double digested and purified, and then connected with each insert and transformed using the DNA Ligation Kit (TaKaRa, China). ..

    Construct:

    Article Title: BaEV-pseudotyped lentiviral vectors enable stable CAR expression and cytotoxic function in NK cells
    Article Snippet: All constructs were synthesized by Lugen Sci Co., Ltd. (Bucheon-si, Gyeonggi-do, Republic of Korea). .. Constructs were cloned into the XbaI and SalI restriction sites of the pCDH-MSCV vector (System Biosciences, Palo Alto, CA, USA) using a DNA Ligation Kit (#6023, Takara Bio, Shiga, Japan). ..

    Article Title: Tracing the emergence of the novel fluoroquinolone resistance gene qrtA in enterococci through environmental reservoirs and pELF-type linear plasmids
    Article Snippet: .. The DNA fragments to be inserted were constructed by PCR using the respective primers and inserted into the pCJK47 vector using the restriction enzyme Eco RI (Roche) and a DNA ligation kit (Takara Bio, Shiga, Japan) as previously described (Table S8) . ..

    Polymerase Chain Reaction:

    Article Title: Tracing the emergence of the novel fluoroquinolone resistance gene qrtA in enterococci through environmental reservoirs and pELF-type linear plasmids
    Article Snippet: .. The DNA fragments to be inserted were constructed by PCR using the respective primers and inserted into the pCJK47 vector using the restriction enzyme Eco RI (Roche) and a DNA ligation kit (Takara Bio, Shiga, Japan) as previously described (Table S8) . ..



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